Figure 7.  During NK cell development in vitro the acquisition of high levels of CD94 and of Qa1 receptors occurs independently of the acquisition of Ly49 molecules
Qa1R				CD94
		4D12				14B11
After 6 days of secondary culture in IL2 developing NK cells were costained with either Qa1 tetramers
and the anti-Ly49 mAb 4D12 [left] or with an anti-CD94 mAb and the anti-Ly49 mAb 14B11[right]
Conclusions
1.  At this stage of development most cells still lack both Qa1R and the Ly49 molecules recognized
by the mAb 4D12
2.  Most of the cells that do express Qa1R do not express Ly49 molecules, and vice versa
3.  The proportion of cells that express both Qa1R and Ly49 molecules is very close the product of
the frequency of cells that express either molecule [the product rule] indicating that these two
sets of molecules are acquired in a stochastic and  independent manner
4.  The same conclusions apply to the acquisition of high levels of CD94 and Ly49 molecules