Figure 9.  CD94/NKG2 receptors can be expressed on developing NK cells in the absence of NK1.1
	A. Fresh thymic progenitors				B. Cultured for 2d in IL4+PMA
NK1.1				NK1.1
		NKG2				NKG2
Early thymic progenitors, either freshly prepared or following primary culture for 2 days in IL4+PMA
were stained with mAbs to TCR, NK1.1, and NKG2 and gated on TCR negative cells
Conclusions
1.  Although freshly prepared E14 thymocytes contain only small numbers of NKG2+ cells,
multicolour staining shows clearly that a substantial proportion of these lack NK1.1
2.  Following primary culture for 2 days in IL4+ PMA there is only a slight increase in the proportion
of cells that express NK1.1 but a more marked increase in the proportion of NKG2+ cells, most of
which are NK1.1-
3.  Similar results are found for CD94, demonstrating that CD94/NKG2 receptors can be expressed
during development in vivo and in vitro in the absence of NK1.1