Figure 10.  Protection of T2Q cells by exogenous Qdm peptide occurs rapidly
Preincubation with
100uM Qdm for:			Qa1R+ NK cells		Qa1-specific CTL clone d12i
0 min
15 min
45 min
120 min
			Lytic units/thousand effectors
T2Q cells were incubated with 100uM Qdm peptide at room temperature for the times shown,
then washed, and incubated with graded doses of Qa1R+ NK cells or with the type 2 Qa1-
specific CTL clone d12i.
Conclusions
1.  A high level of protection of T2Q cells from lysis by Qa1R+ NK cells is achieved within 15 min of
exposure of T2Q cells to exogenous Qdm peptide, making it highly likely that the protective
Qa1-Qdm complexes are formed at the cell surface.
2.  In the same time period [15 min] maximal sensitivity to lysis by CTL is achieved.