| Figure 11. The protected state induced by incubation
of T2Q cells with Qdm peptide is highly stable |
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Qa1R+ NK cells |
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Qa1-specific CTL clone d12i |
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Lytic units/thousand effectors |
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| T2Q cells were
incubated overnight at 26o with or without 100uM Qdm peptide then washed |
| and incubated
for the times shown at 37o before adding to wells containing graded doses of |
| Qa1R+ NK cells or the
type 2 Qa1-specific CTL clone d12i. |
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| Conclusions |
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| 1. The protected state was fully maintained
for 4hr at 37o in the absence of Qdm peptide, but was |
| lost after
7hr. |
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| 2. The state of sensitivity to Qa1-specific
CTL was still maximal after 7hr, indicating that much |
| lower
concentrations of surface Qa1-Qdm complexes are required for sensitivity to
CTL killing than |
| for protection
from NK cell killing |
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