Figure 11.  The protected state induced by incubation of T2Q cells with Qdm peptide is highly stable
Qa1R+ NK cells Qa1-specific CTL clone d12i
Lytic units/thousand effectors
T2Q cells were incubated overnight at 26o with or without 100uM Qdm peptide then washed
and incubated for the times shown at 37o before adding to wells containing graded doses of
Qa1R+ NK cells or the type 2 Qa1-specific CTL clone d12i.
Conclusions
1.  The protected state was fully maintained for 4hr at 37o in the absence of Qdm peptide, but was
lost after 7hr.
2.  The state of sensitivity to Qa1-specific CTL was still maximal after 7hr, indicating that much
lower concentrations of surface Qa1-Qdm complexes are required for sensitivity to CTL killing than
for protection from NK cell killing