Figure 4.  Despite their very high levels of surface Qa1 molecules, T2Q cells are not protected from lysis by Qa1R+ NK cells
			NK clone J1.1		NK clone I7
			Qa1R +ve		Qa1R -ve
		T2
		T2-Ld
		T2Q
		L
		LQ
			Lytic units/thousand effectors
T2 cells transfected with Qa1 [T2Q] were incubated with graded doses of Qa1R+ or Qa1R- NK cells
and susceptibility to lysis calculated by regression analysis of the linear part of the dose-response.
Controls included untransfected T2 cells, T2 cells transfected with Ld, L cells, and L cells
transfected with Qa1.
Conclusions
1.  T2 cells transfected with Qa1 are as susceptible to killing by Qa1R+ NK cells as untransfected
or control transfected cells
2.  By contrast, L cells transfected with Qa1 are highly resistant to killing by Qa1R+ NK cells
3.  The Qa1 molecules expressed on T2Q cells cannot protect them from killing by Qa1R+ NK cells,
ie. are not recognized by the inhibitory Qa1 receptors expressed on these cells
4.  The implication is that only Qa1 molecules containing appropriate peptides in their grooves are
protective