| Figure 6. TAP-deficient T2Q cells, but not
TAP-deficient RMA/S cells, can be protected from lysis by Qa1R+ NK cells
following pre-incubation with Qdm peptide |
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NK clone J1.1 |
NK clone I7 |
Type 1 CTL |
Type 2 CTL |
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Qa1R +ve |
Qa1R -ve |
clone a2d |
clone d12i |
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| T2Q -Qdm |
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| T2Q +Qdm |
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| RMA/S -Qdm |
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| RMA/S +Qdm |
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| RMA |
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Lytic units/thousand effectors |
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| T2Q and RMA/S
cells were incubated overnight at 26o with or witout 100uM Qdm peptide, |
| mixed
with graded doses of NK or CTL effector cells, and susceptibility to lysis
calculated by |
| regression
analysis of the linear part of the dose-response. |
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| Conclusions |
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| 1. Preincubation with Qdm peptide renders T2Q
cells resistant to lysis by Qa1R+ but not |
| Qa1R- NK cells |
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| 2. Preincubation with Qdm peptide also
renders T2Q cells sensitive to lysis by the type 2 CTL clone |
| d12i but
causes no change in their sensitivity to lysis by type 1 CTL clones |
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| 3. By contrast, preincubation with Qdm
peptide has no effect on the sensitivity of RMA/S cells to |
| lysis
by NK cells, even though it renders them highly sensitive to lysis by both
type 1 and type 2 |
| Qa1-specific
CTL. |
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