Figure 9.  Protection of T2Q cells by inclusion of peptide in the cytotoxicity assay occurs at peptide concentrations similar to or lower than those required for protection following overnight preloading
	[Qdm]		Preloading			Inclusion
	0
	1 nM
	10 nM
	100 nM
	1 mM
	10 mM
	100 mM
			Lytic units/thousand effectors
T2Q cells were loaded with various concentrations of Qdm peptide either by incubating them
overnight at 26o in serum-free medium, followed by washing, or by adding peptide to them just
prior to mixing them with Qa1R+ NK cells
Conclusions
The dose-response curve for peptide-mediated protection from NK cell lysis is similar under both
conditions, but protection is initially seen at a lower peptide concentration [10nM] in the inclusion
assay than in the preloading assay.