Figure 11.  Structure of murine IL15Ra gene and mRNA

Gene Locus

Chromosome 2, LocusLink 16169, LOC227570

Ensembl Gene ENSMUSG00000023206

Exons shown in blue

Isoform 1 and Isoform 2 mRNA

GACAGCATTAAGGAATGAAAAGGGAGATCGCCGGCTTCTACGG

GAGCTGAGCTCGCCACCTCCCAGGGACCCCAGCCTTGCGTCCCGTTGGGTCACTGCTGGGGACAATTGGC

CATGGCCTCGCCGCAGCTCCGGGGCTATGGAGTCCAGGCCATTCCTGTGTTGCTGCTGCTGCTGTTGCTA

CTGTTGCTCCCGCTGAGGGTGACGCCGGGCACCACGTGTCCACCTCCCGTATCTATTGAGCATGCTGACA

TCCGGGTCAAGAATTACAGTGTGAACTCCAGGGAGAGGTATGTCTGTAACTCTGGCTTTAAGCGGAAAGC

TGGAACATCCACCCTGATTGAGTGTGTGATCAACAAGAACACAAATGTTGCCCACTGGACAACTCCCAGC

CTCAAGTGCATCAGAGACCCCTCCCTAGCTCACTACAGTCCAGTGCCAACAGTAGTGACACCAAAGGTGA

CCTCACAGCCAGAGAGCCCCTCCCCCTCTGCAAAAGAGCCAGAAGCTTTCTCTCCCAAATCAGATACCGC

AATGACCACAGAGACAGCTATTATGCCTGGCTCCAGGCTGACACCATCCCAAACAACTTCTGCAGGAACT

ACAGGGACAGGCAGTCACAAGTCCTCCCGAGCCCCATCTCTTGCAGCAACAATGACCTTGGAGCCTACAG

CCTCCACCTCCCTCAGGATAACAGAGATTTCTCCCCACAGTTCCAAAATGACGAAAGTGGCCATCTCTAC

ATCGGTCCTCTTGGTTGGTGCAGGGGTTGTGATGGCTTTCCTGGCCTGGTACATCAAATCAAGGCAGCCT

TCTCAGCCGTGCCGTGTTGAGGTGGAAACCATGGAAACAGTACCAATGACTGTGAGGGCCAGCAGCAAGG

AGGATGAAGACACAGGAGCCTAACCACACGATTTCAGAAACTCAGGGAGACCTGCCCAGCTGATCCTGAA

GAGAAGATACAAGGGCAGTTAGTCCAGCTGCGCCTGGACACAGGAGAAAGCCCAATGTGATAATAGAGGT

CTCTGGTATGATCTGTTTTACTGAGCCTGGGTGCCTCCCATATTCAGGCCTGGATCCCCGGAGTCCGGAA

GGACACCACCTTTCTTCCTGGTTCATCACAGAGGCCAACTTCCCAGAGTACAAGCAGCCTGAGCTCTCCT

GGGAGTCTCCATTAAAAGCCTCGAGTTCCACTCAGAGATTAACTCAAAGCAGCTGATCTGCTAGACTCTT

TTTCTATTCCCTACTCTGACTTGCAGTTTACAGAGATTGACAAGGCTCCCATTGTCTTCCAAGGCTCCTC

TGGCACAGGAGATGTCTGTAAAGAAGACAGCAACATTTGAGCTCCTGAAGACTTGGCCCTTTGCTGCTTT

GCACCTATTGGAGGAGAGCAGAGAACAGAAGAAGAGATACTGAGCCAATGAACCCTTTCGTATAGGATTC

ATGACAAAACCAAACTCAGTGACTATATATGTATGTGTGTGTGTGTGTGTATGTAAAAGTGTATATTTAC

ATATACATTTATATTTATACTTTCTTTTCTATTATATCTACATATTGTATATGATTTATATTTGAAAGTG

CTTTGTGTAGACAAAATAAAATATCTATTTTCAGTACAAAAAGCATTAAAATTATCAACA

Notes

1.  The first base of exon -1 corresponds to nucleotide 1,194,228 of supercontig NW_000173

NW_000173

2. The start codon is located at 11,789,345 and the stop codon at 11,817,601 of Ensembl v9.3a1 of the mouse

genome.

3.  Exons are shown in alternate blue and green letters except exon 1 and the alternate exon -1 [shown separately

above] in brown.

4.  The 1600 base mRNA that contains exon 1 corresponds to Genbank sequence MMU22339 elucidated by

Giri et al and encodes the 263 amino acid IL15Ra Isoform 1 with the translational start codon in exon 1 and

termination codon in exon 7.

U22339

Giri et al.

5.  Replacement of exon 1 with exon -1 generates the transcript corresponding to Genbank sequence

NM_133836 deposited by Giri et al that encodes the 123 amino acid IL15Ra Isoform 2 with the translational

start codon in exon 4 and termination codon in exon 7.

NM 133836

6.  Translational start and stop codons are shown in red letters.

7.  The red G in exon 4 is the cryptic splice acceptor site that splices to the end of exon 3 in Isoform 1A

[see Figure 13].

8.  Primer target sequences used for PCR are underlined.