| Figure 14.
Expression of IL2Ra chains on NK cells and T cells |
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A. Immature NK cells in IL2 |
F. NK line 1608b in IL2 |
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B. Immature NK cells in IL15 |
G. NK line I2/22 in IL2 |
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H. CD4 T cells in IL2 |
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D. Mature NK cells in IL2 |
I. CD8 T cells in IL2 |
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E. Mature NK cells in IL15 |
J. CTLL2 in IL2 |
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| The cells
described were stained with medium [black] or biotin-conjugated anti-IL2Ra
mAb [red] |
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Alexa Fluor 647 streptavidin. |
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| Conclusions |
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| 1. In agreement with the finding of IL2Ra
mRNA in NK cells, both immature and mature NK cells |
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| were found to
express detectable levels of IL2Ra at the cell surface |
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| 2. However, the levels expressed by freshly
obtained NK cells in culture are low, being about one |
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| tenth of the
levels found on CD4 and CD8 ConA-induced blasts, and about hundredth of the
levels |
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| expressed on
CTLL2 cells. |
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| 3. They were also only detectable on freshly
obtained NK cells when these were grown in IL15. |
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| 4. By contrast, the established NK cell lines
I22/22 and 1608b expressed readily detectable levels |
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| of surface IL2Ra
chains even when grown in IL2. |
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