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EspF EPEC secreted protein F

EspF Protein Sequence of EPEC strain E2348/69
pubmed

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MLNGISNAASTLGRQLVGIASRVSSAGGTGFSVAPQAVRLTPVRVHSPFSPGSSNVNARTIFNVSSQVT SFTPSRPAPPPPTSGQASGASRPLPPIAQALKDHLAAYELSKASETVNFKPTRPAPPPPTSGQASGASR PLPPIAQALKDHLAAYELSKASETVSFKPTRQAPPPPTSGQASGPGGLPPLAQALKDHLAAYEQSKKG

EspF Publications

Homologues

Secondary Structure.pdf

EspF Features

Colour Alignment

Phylogenetic Tree

REPORTED FUNCTIONS

Actin-Pedestal Elongation, Tight Junction disruption, Mitochondrial dysfunction, Apoptosis, Microvilli Effacement, Microvilli Elongation, Actin nucleation, Anti-phagocytosis. Causes mis-localisation of aquaporins

PROVEN BINDING PARTNERS

Cytokeratin 18, 14-3-3, ABCF2, N-WASP, Sorting Nexin 9, Actin

SUBCELLULAR LOCATIONS

Mitochondria, plasma membrane, cytoplasm, nucleolus

PHYSICO-CHEMICAL

206 amino acids, 20.9 kDa, pI 10.61, 3 Proline-Rich Repeats

DISCOVERED BY

Michael Donnenberg’s Lab (McNamara and Donnenberg 1998)

FUNCTIONAL MOTIFS

3 Sorting Nexin 9 binding motifs (Alto et al 2007; Marches et al 2006)

3 N-WASP binding motifs (Alto et al 2007)

Mitochondrial targeting sequence at residues 1-25 (Nagai et al 2005)

A 14-3-3 binding motif (linked but not yet proven; Viswanathan et al 2004)

EspF Proline Rich Repeat regions  

Amino Acid Position

Sorting nexin 9 binding motif

N-WASP binding motif
repeats

DNA Sequence of the espF gene of EPEC strain E2348/69  

ATGCTTAATGGAATTAGTAACGCTGCTTCTACACTAGGGCGGCAGCTTGTAGGTATCGCAAGTCGAGTGA
GCTCTGCGGGGGGAACTGGATTTTCTGTAGCCCCTCAGGCTGTTCGTCTTACTCCGGTGAGAGTTCATTC
CCCCTTTTCTCCAGGTTCGTCGAATGTTAATGCGAGGACGATTTTTAATGTGAGCAGCCAGGTGACTTCA
TTTACTCCCTCTCGTCCGGCACCGCCGCCACCGACAAGTGGACAGGCATCCGGGGCATCCCGACCTTTAC
CGCCCATTGCACAGGCACTAAAAGATCATTTAGCTGCCTATGAACTATCGAAAGCGTCTGAGACTGTAAA
CTTTAAGCCAACCCGTCCGGCACCGCCGCCACCGACAAGTGGACAGGCATCCGGGGCATCCCGACCTTTA
CCGCCCATTGCACAGGCATTAAAAGATCATTTAGCTGCCTATGAACTATCGAAAGCGTCTGAGACTGTAA
GCTTTAAGCCAACCCGTCAGGCACCACCGCCACCGACAAGTGGACAGGCATCCGGGCCTGGTGGTCTACC
GCCCCTTGCACAGGCACTAAAAGATCATTTAGCTGCCTATGAGCAATCGAAGAAAGGGTAA

Genomic sequence - upstream and downstream of espF 

TATTATCTTATTTGGATTAAATAGAAAAAATGATGCGATAAAGGCTTTGGAGGGCTGTATGGATGATGAGTC GAGTCAGTTATTGTACAGTTTGTTCCATGAGAATGTTAGTGGTTGGGTACGAGGATTTTAATTTATTTTGAT TTAAAAATCATTTTATTATTCGTTTTGATATATATGAGAGTTAGCCAAGATTAGATATAAAGAGGCATAAAT TATGCTTAATGGAATTAGTAACGCTGCTTCTACACTAGGGCGGCAGCTTGTAGGTATCGCAAGTCGAGTGAG CTCTGCGGGGGGAACTGGATTTTCTGTAGCCCCTCAGGCTGTTCGTCTTACTCCGGTGAGAGTTCATTCCCC CTTTTCTCCAGGTTCGTCGAATGTTAATGCGAGGACGATTTTTAATGTGAGCAGCCAGGTGACTTCATTTAC TCCCTCTCGTCCGGCACCGCCGCCACCGACAAGTGGACAGGCATCCGGGGCATCCCGACCTTTACCGCCCAT TGCACAGGCACTAAAAGATCATTTAGCTGCCTATGAACTATCGAAAGCGTCTGAGACTGTAAACTTTAAGCC AACCCGTCCGGCACCGCCGCCACCGACAAGTGGACAGGCATCCGGGGCATCCCGACCTTTACCGCCCATTGC ACAGGCATTAAAAGATCATTTAGCTGCCTATGAACTATCGAAAGCGTCTGAGACTGTAAGCTTTAAGCCAAC CCGTCAGGCACCACCGCCACCGACAAGTGGACAGGCATCCGGGCCTGGTGGTCTACCGCCCCTTGCACAGGC ACTAAAAGATCATTTAGCTGCCTATGAGCAATCGAAGAAAGGGTAACCAACTAATTTAAAACGATTAATCGG TATTGATTATTTGTTTTCCAACTAATAAAAACCGATATCAAATTGCGTGGTAATAAATGTGATTAGTAATAT CGGTTTTTATTGGAGGGGTAATAAATAATGTATTATTATACGGTGAAAGTGGAGAAAGAGACGTTTTGTTAA AAGCGCACAGAGCTCAAAAGTCTGTTCGCTATTGATTGTGATAATCTGAAAGGCAGTAAACTCAGGTATACT GGCAATAGCGGACACCACCATTTGTTC