Effector Link
[EspZ] [Map] [Tir] [EspH] [EspB] [EspF]

EspG EPEC secreted protein G

EspG Protein Sequence of EPEC strain E2348/69
pubmed

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NMPDRjpeg 
MILVAKLFITNQIGESLMINGLNNDSASLVLDAAMKVNSGFKKSWDEMSCAEKLLKVLSFGLWNPTYSRS
ERQSFQELLTVLEPVYPLPNELGRVSARFSDGSSLRISVTNSESIEAEIRTPDNEKITVLLESNEQNRLL
QSLPIDRHMPYIQVHRALSEMDLTDTTSMRNLLGFTSKLSTTLIPHNAQTDPLSGPTPFSSIFMDTCRGL
GNAKLSLNGVDIPANAQMLLRDALGLKDTHSSPSRNVIDHGISRHDAEQIARESSGSDNQKAEVVEFLCH
PEAATAICSAFYQSFNVPALTLTHERISKASEYNAERSLDTPNACINISISQSSDGNIYVTSHTGVLIMA
PEDRPNEMGMLTNRTSYEVPQGVKCTIDEMVRALQPRYAASETYLQNT

Homologues

Secondary Structure.pdf

Colour Alignment

EspG Features

Phylogenetic Tree

REPORTED FUNCTIONS

Plays a minor role in epithelial barrier dysfunction. Interacts with tubulin and disrupts microtubules. Causes actin stress fibre formation. Inhibits intestinal chloride  transporter. Causes mis-localisation of aquaporins

PROVEN BINDING PARTNERS

Tubulin

PHYSICO-CHEMICAL

398 amino acids, 43.9 kDa, pI 5.21

DISCOVERED BY

James Kaper’s Lab (Elliot et al 2001)

HOMOLOGUES

EspG2(Orf3), VirA (Shigella effector)

DNA sequence of the espG gene of EPEC strain E2348/69  

ATGATACTTGTTGCCAAATTGTTCATTACAAACCAGATAGGAGAATCTCTCATGATAAATGGACTTAATA
ATGACTCCGCATCTTTAGTTTTAGATGCTGCAATGAAAGTTAATTCTGGGTTTAAAAAAAGCTGGGATGA
GATGTCATGCGCTGAAAAGTTACTTAAAGTACTTAGTTTTGGTTTATGGAATCCAACGTACAGTCGTAGT
GAAAGACAATCATTTCAAGAGTTGTTAACCGTTTTAGAGCCTGTATATCCACTTCCCAATGAATTAGGCA
GAGTATCTGCTCGTTTTTCAGATGGCTCATCCTTAAGAATTTCCGTCACTAACAGCGAAAGTATTGAAGC
CGAGATTCGCACACCAGATAATGAAAAGATTACTGTGCTCCTGGAGTCAAACGAACAAAATAGGTTATTA
CAATCTTTACCCATCGATCGCCACATGCCATACATTCAGGTTCATCGTGCCTTATCTGAGATGGACCTGA
CTGATACTACCTCAATGCGCAATCTACTTGGTTTTACGTCAAAACTATCAACAACCTTGATTCCTCATAA
TGCTCAAACAGATCCGCTTTCCGGGCCTACACCATTCAGCTCTATCTTTATGGATACATGTCGAGGACTC
GGCAATGCAAAGCTTTCACTCAATGGTGTTGATATACCTGCAAATGCACAAATGTTGCTTCGCGATGCAC
TAGGACTTAAAGACACACACTCATCACCTTCCCGGAATGTTATAGATCATGGTATTTCTCGCCATGATGC
AGAGCAAATAGCAAGAGAAAGCAGCGGCAGCGATAATCAGAAAGCTGAAGTTGTGGAATTTTTATGCCAT
CCAGAAGCAGCAACGGCCATATGCTCGGCTTTCTATCAATCTTTCAATGTGCCAGCCTTAACGTTGACAC
ATGAAAGGATCTCTAAAGCCAGTGAATACAATGCGGAAAGATCGTTAGATACACCTAACGCTTGCATTAA
CATCAGTATCTCTCAATCATCAGATGGAAACATTTATGTTACCAGCCATACCGGGGTTCTGATAATGGCG
CCAGAAGACCGCCCCAACGAGATGGGCATGTTGACGAACAGGACTTCTTATGAAGTGCCGCAAGGTGTGA
AATGTACAATCGATGAAATGGTAAGAGCGCTACAACCAAGGTATGCCGCATCTGAAACGTACTTACAAAA
CACTTAA

 

Genomic sequence - upstream and downstream of espG 

AGTATTGCCCATATCCATTCGGCGAAAGAGGATCTGCCATACATCAAGTCCGTTTTACTTGGCATCAGATCATCGCCGTCATT AACTCTGTTAAAGTCGGACAAACGATTAGCAAGATCTGTGATTCAAAAGTGCATAGCGCTAAGGTGGGACTGGTTACAAAT TCATTAACCCTGTTCTTTGTCGCACCTATCGATCGGTGTAATGCCACAACAGGTTTATGTTAATCAGAACAATGCGCCCCTCT ATAAAGAGAATTCTTTGATAAGGCAACTTGAAAAGGGAAAGCTAACTGATATCAATGTACCACTTTAGTAATCATCACATTA TATACAGGCCAAAATAAGTTTCCTGAAAGCCAGTATCTCTAAATCAATCATTAATGGTATATTTTATCGTCACAATAAAAAA TTATCTAAATTCTATTACTTATGTAAATAAGTACAACCTTTTCATAGACACAAAAACTATGGCTGACGCATCACTTGCAATCA ATTATCCATCATTAGATTAATATTTTCACTTATTTTTCAGAAGGTAAAAATAACTTTCTATGCGTTTAAAACACCCGCTATAAA TATCTTGTATATTATTGACACTATATGATGCTATATGATACTTGTTGCCAAATTGTTCATTACAAACCAGATAGGAGAATCTCT CATGATAAATGGACTTAATAATGACTCCGCATCTTTAGTTTTAGATGCTGCAATGAAAGTTAATTCTGGGTTTAAAAAAAGCT GGGATGAGATGTCATGCGCTGAAAAGTTACTTAAAGTACTTAGTTTTGGTTTATGGAATCCAACGTACAGTCGTAGTGAAAG ACAATCATTTCAAGAGTTGTTAACCGTTTTAGAGCCTGTATATCCACTTCCCAATGAATTAGGCAGAGTATCTGCTCGTTTTTC AGATGGCTCATCCTTAAGAATTTCCGTCACTAACAGCGAAAGTATTGAAGCCGAGATTCGCACACCAGATAATGAAAAGAT TACTGTGCTCCTGGAGTCAAACGAACAAAATAGGTTATTACAATCTTTACCCATCGATCGCCACATGCCATACATTCAGGTTC ATCGTGCCTTATCTGAGATGGACCTGACTGATACTACCTCAATGCGCAATCTACTTGGTTTTACGTCAAAACTATCAACAACC TTGATTCCTCATAATGCTCAAACAGATCCGCTTTCCGGGCCTACACCATTCAGCTCTATCTTTATGGATACATGTCGAGGACTC GGCAATGCAAAGCTTTCACTCAATGGTGTTGATATACCTGCAAATGCACAAATGTTGCTTCGCGATGCACTAGGACTTAAAG ACACACACTCATCACCTTCCCGGAATGTTATAGATCATGGTATTTCTCGCCATGATGCAGAGCAAATAGCAAGAGAAAGCA GCGGCAGCGATAATCAGAAAGCTGAAGTTGTGGAATTTTTATGCCATCCAGAAGCAGCAACGGCCATATGCTCGGCTTTCTA TCAATCTTTCAATGTGCCAGCCTTAACGTTGACACATGAAAGGATCTCTAAAGCCAGTGAATACAATGCGGAAAGATCGTTA GATACACCTAACGCTTGCATTAACATCAGTATCTCTCAATCATCAGATGGAAACATTTATGTTACCAGCCATACCGGGGTTCT GATAATGGCGCCAGAAGACCGCCCCAACGAGATGGGCATGTTGACGAACAGGACTTCTTATGAAGTGCCGCAAGGTGTGA AATGTACAATCGATGAAATGGTAAGAGCGCTACAACCAAGGTATGCCGCATCTGAAACGTACTTACAAAACACTTAAAATA AAACTTTATTAATCAAAACCAATAATAGAAATCACACCCACAATTATTAACAATTTATCCTGAAGTTAATTACAATCAAACA AAAATATTTGTTCTGCAAACAATTTACAGTAAATAGAGAAAATGAAAAGAAAACCATTAATTGCATTTTTCATTGCGATATT TATTATGCTAACCATATTAATATTATTTCCATTTAATTGTACTTACAAAAAATCACCTCAAGAACACTCACTTTCATTTATAAA ACAATTACCAAATACGGTGAACTTATCATCTCTTACTTATAATAAAGAGGATGATTTCCTGTATGCCACCCAGAATAGCCCTG CACAGCTACTTAAAATAACCAAAAGTGGCGACATCATGGACAGAGCCCCGCTGCCTTTTATCTCTGATGCTGAAACAATTGA ACACATACAAGGGAACATTTTTGCTGCAGTAGACGAAAAGACTTCTGAACTATTCTTTTTTACAGTTACAAAAGATATGCAT ATCTCCTTTCGAAATAAAATTCAGTTAGAAAAATTCAATAAAAAGAATAGAGGGTTTGAAGGGCTGGCATGGAAAGCAGAT GATCGGATGCTGTTTGTCGCAAAAGAAAGACGCCCAAGTAAAATATTTATATATCAACTTTCTTCAGACCTGCTGAGTGTAA AGCAAGCAACTATTCCAGAGG