Effector Link
[EspZ] [Map] [EspG] [EspH] [EspB] [EspF]

Tir Translocated Intimin Receptor

Tir Protein Sequence of EPEC strain E2348/69
pubmed

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NMPDRjpeg

MPIGNLGNNVNGNHLIPPAPPLPSQTDGAARGGTGHLISSTGALGSRSLFSPLRNSMADSVDSRDIPGLP TNPSRLAAATSETCLLGGFEVLHDKGPLDILNTQIGPSAFRVEVQADGTHAAIGEKNGLEVSVTLSPQEW SSLQSIDTEGKNRFVFTGGRGGSGHPMVTVASDIAEARTKILAKLDPDNHGGRQPKDVDTRSVGVGSA SGIDDGVVSETHTSTTNSSVRSDPKFWVSVGAIAAGLAGLAATGIAQALALTPEPDDPTTTDPDQAANA AESATKDQLTQEAFKNPENQKVNIDANGNAIPSGELKDDIVEQIAQQAKEAGEVARQQAVESNAQAQ QRYEDQHARRQEELQLSSGIGYGLSSALIVAGGIGAGVTTALHRRNQPAEQTTTTTTHTVVQQQTGGNT PAQGGTDATRAEDASLNRRDSQGSVASTHWSDSSSEVVNPYAEVGGARNSLSAHQPEEHIYDEVAADP GYSVIQNFSGSGPVTGRLIGTPGQGIQSTYALLANSGGLRLGMGGLTSGGESAVSSVNAAPTPGPVRFV

Homologues

Colour Alignment

Secondary Structure.pdf

Tir Features

Phylogenetic Tree

REPORTED FUNCTIONS

Nucleates and polymerises actin; regulator the functions of other effectors such as Map, EspF and EspG; downregulates filopodia; promotes cell invasion; a receptor for the bacterial outer membrane protein Intimin; Plays a role in effacement; Suppresses cell detachment; PLC activation.

 BINDING PARTNERS

IQGAP, Nck, alpha-actinin, vinculin, talin cortactin, CK18

PHYSICO-CHEMICAL

550 amino acids, 56.5 kDa, pI 4.92

DISCOVERED BY

Brett Finlay’s Lab (Kenny et al 1997)

HOMOLOGUES

no substantial homology to other proteins

DNA sequence of the tir gene of EPEC strain E2348/69  

ATGCCTATTGGTAACCTTGGTAATAATGTAAATGGCAATCATTTAATTCCCCCTGCGCCGCCACTACCTT
CACAAACAGACGGCGCGGCACGGGGAGGAACTGGTCATCTAATTAGCTCTACAGGAGCATTAGGATCTCG
TTCATTGTTTTCTCCCCTGAGAAATTCTATGGCTGATTCTGTCGATTCCAGAGATATTCCAGGACTTCCT
ACAAACCCATCGAGGCTTGCTGCAGCTACATCTGAGACATGCTTGCTTGGAGGATTTGAAGTTCTCCATG
ATAAGGGGCCACTTGATATTCTCAATACGCAAATTGGACCCTCTGCATTTCGTGTTGAAGTGCAGGCAGA
TGGTACTCATGCCGCTATTGGAGAAAAAAATGGTTTGGAGGTTAGCGTTACATTAAGTCCTCAAGAATGG
AGCAGCTTGCAATCTATTGATACTGAGGGTAAAAACAGATTTGTTTTTACCGGGGGACGTGGCGGTAGTG
GGCATCCGATGGTCACTGTCGCATCAGATATCGCGGAAGCTCGTACGAAAATACTGGCCAAATTAGACCC
AGACAATCATGGAGGACGTCAACCCAAGGACGTTGATACGCGTTCTGTTGGTGTTGGCAGCGCTTCGGGA
ATAGATGATGGCGTTGTTAGCGAAACCCATACTTCAACAACAAATTCCAGCGTTCGCTCAGATCCTAAAT
TCTGGGTTTCTGTCGGCGCAATTGCTGCTGGTTTAGCGGGACTGGCGGCAACTGGTATTGCACAGGCGTT
GGCTTTGACACCGGAACCGGATGATCCTACAACCACCGATCCTGATCAGGCCGCAAATGCTGCAGAAAGT
GCAACAAAAGATCAGTTAACGCAAGAAGCATTCAAGAACCCTGAGAACCAGAAAGTTAACATCGATGCGA
ACGGAAATGCTATTCCGTCTGGGGAATTAAAAGATGATATTGTTGAGCAAATAGCACAACAAGCTAAAGA
GGCTGGTGAGGTGGCCAGACAGCAGGCTGTTGAAAGCAATGCACAGGCGCAGCAGCGATATGAGGATCAG
CATGCCAGACGTCAGGAGGAATTACAGCTTTCATCGGGTATTGGTTACGGCCTCAGCAGTGCATTGATTG
TTGCTGGGGGAATTGGTGCTGGTGTAACGACTGCGCTCCATAGACGAAATCAGCCGGCAGAACAGACAAC
TACTACAACAACACATACGGTAGTGCAGCAACAGACCGGAGGGAATACCCCAGCACAAGGTGGCACTGAT
GCCACAAGAGCAGAAGACGCTTCTCTGAATAGACGTGATTCGCAGGGGAGTGTTGCATCGACACACTGGT
CAGATTCCTCTAGCGAAGTGGTTAATCCATATGCTGAAGTTGGGGGGGCTCGGAATAGTCTATCGGCTCA
TCAGCCAGAAGAGCATATTTATGATGAGGTCGCTGCAGATCCTGGTTATAGCGTTATTCAGAATTTTTCA
GGGAGCGGCCCAGTTACCGGAAGGTTAATAGGAACTCCAGGGCAAGGTATCCAAAGTACTTATGCGCTTC
TGGCAAACAGCGGCGGATTGCGTTTAGGTATGGGAGGATTAACGAGTGGTGGCGAGAGCGCAGTAAGTTC
TGTAAATGCCGCACCAACGCCGGGACCAGTACGTTTCGTTTAA
 

Genomic sequence - upstream and downstream of tir  

ATGTCGCATTTTTAAAGAGGTTGAAAGCGCGATACAACAGCCTCTCGATATGAACTGTGCACAATCATCA
ATTAGTCACTTTTTGCAATCCAATAAATACTTTAATCAGAAAGTTGATGAGCAGTGTGGTAAAGGAGTGG
ATCCCATTACACGTTTTAACACTCAAACCAAATTGATAGAACAAGTCTCACGCGAAATCTTTGAACAGAA
TTTTAGCACCGCGAAAATCAGTGATATCAAGGCTCTAACTGAGAAGGCGATTGCAGACAATGTGCAGGAT
ACTCGGCTGTAGGGGGAAACTTACTGCGCTGTTATTTTTTTTCTTGATGCAAAAAGGTCTCTATAGACGT
TTAAAATAAAATATTATTTTTCAATACACAATTAAATTTCTGCATATAAAAATTATAATTATTTTTTTTC
GATAATTATATAATGATTTTGATTATGTGATTTTTTAGTTGGAAATACAGACATGCATTTCTGGTGCGTT
ATTTTGCTTGCATCAAAAATTATACTGTGATTTATTTGGTTTATGCTCAGTTGTTTTATCGGCTGCATAC
CGTTACGTCATAGTAATATAAAGGAACGTGTCAAATTTCTAAATAAAAGGATATATGTATGCCTATTGGT
AACCTTGGTAATAATGTAAATGGCAATCATTTAATTCCCCCTGCGCCGCCACTACCTTCACAAACAGACG
GCGCGGCACGGGGAGGAACTGGTCATCTAATTAGCTCTACAGGAGCATTAGGATCTCGTTCATTGTTTTC
TCCCCTGAGAAATTCTATGGCTGATTCTGTCGATTCCAGAGATATTCCAGGACTTCCTACAAACCCATCG
AGGCTTGCTGCAGCTACATCTGAGACATGCTTGCTTGGAGGATTTGAAGTTCTCCATGATAAGGGGCCAC
TTGATATTCTCAATACGCAAATTGGACCCTCTGCATTTCGTGTTGAAGTGCAGGCAGATGGTACTCATGC
CGCTATTGGAGAAAAAAATGGTTTGGAGGTTAGCGTTACATTAAGTCCTCAAGAATGGAGCAGCTTGCAA
TCTATTGATACTGAGGGTAAAAACAGATTTGTTTTTACCGGGGGACGTGGCGGTAGTGGGCATCCGATGG
TCACTGTCGCATCAGATATCGCGGAAGCTCGTACGAAAATACTGGCCAAATTAGACCCAGACAATCATGG
AGGACGTCAACCCAAGGACGTTGATACGCGTTCTGTTGGTGTTGGCAGCGCTTCGGGAATAGATGATGGC
GTTGTTAGCGAAACCCATACTTCAACAACAAATTCCAGCGTTCGCTCAGATCCTAAATTCTGGGTTTCTG
TCGGCGCAATTGCTGCTGGTTTAGCGGGACTGGCGGCAACTGGTATTGCACAGGCGTTGGCTTTGACACC
GGAACCGGATGATCCTACAACCACCGATCCTGATCAGGCCGCAAATGCTGCAGAAAGTGCAACAAAAGAT
CAGTTAACGCAAGAAGCATTCAAGAACCCTGAGAACCAGAAAGTTAACATCGATGCGAACGGAAATGCTA
TTCCGTCTGGGGAATTAAAAGATGATATTGTTGAGCAAATAGCACAACAAGCTAAAGAGGCTGGTGAGGT
GGCCAGACAGCAGGCTGTTGAAAGCAATGCACAGGCGCAGCAGCGATATGAGGATCAGCATGCCAGACGT
CAGGAGGAATTACAGCTTTCATCGGGTATTGGTTACGGCCTCAGCAGTGCATTGATTGTTGCTGGGGGAA
TTGGTGCTGGTGTAACGACTGCGCTCCATAGACGAAATCAGCCGGCAGAACAGACAACTACTACAACAAC
ACATACGGTAGTGCAGCAACAGACCGGAGGGAATACCCCAGCACAAGGTGGCACTGATGCCACAAGAGCA
GAAGACGCTTCTCTGAATAGACGTGATTCGCAGGGGAGTGTTGCATCGACACACTGGTCAGATTCCTCTA
GCGAAGTGGTTAATCCATATGCTGAAGTTGGGGGGGCTCGGAATAGTCTATCGGCTCATCAGCCAGAAGA
GCATATTTATGATGAGGTCGCTGCAGATCCTGGTTATAGCGTTATTCAGAATTTTTCAGGGAGCGGCCCA
GTTACCGGAAGGTTAATAGGAACTCCAGGGCAAGGTATCCAAAGTACTTATGCGCTTCTGGCAAACAGCG
GCGGATTGCGTTTAGGTATGGGAGGATTAACGAGTGGTGGCGAGAGCGCAGTAAGTTCTGTAAATGCCGC
ACCAACGCCGGGACCAGTACGTTTCGTTTAAATATATCTGTGAGTATTTAGTTGAGGTTGGGGTGGGGTG
GGGGGGCGTTTTACTAGCGTTAATGTTTCAGAGAACAACGTTGCAGCATGGGTAACTCTTGAACTTCTGT
TATTATAAATCAATTAAGAGAAATTATAATGTCATCAAGATCTGAACTTTTATTAGATAGGTTTGCGGAA
AAAATTGGTGTTGGATCTATTTCCTTTAATGAAAATAGATTGTGTTCTTTTGCTATTGATGAAATTTATT
ATATTTCGTTATCTGATGCCAATGACGAATATATGATGATTTATGGCGTCTGTGGAAAATTCCCGACAGA
TAACCCTAACTTCGCTCTTGAGATTTTGAATGCAAACTTATGGTTTGCAGAGAATGGTGGGCCATACCTG
TGTTATGAGTCAGGAGCACAATCGCTGTTGTTAGCGTTACGTTTCCCGCTCGATGATGCTACCCCTGAAA
AACTTGAGAATGAAATAGAAGTCGTCGTTAAGTCGATGGAAAATCTGTATTTGGTATTACATAATCAGGG
AATAACATTAGAAAACGAACATATGAAAATAGAGGAAATTAGCTCAAGCGATAATAAACATTATTACGCC
GGAAGATAAAACTTATTTATTAGTATTAATTTACTACTCATTCTAACTCATTGTGGTGGAGCCCAAACAT
GATTACTCATGGTTTTTATGCCCGGACCCGGCACAAGCATAAGCTAAAAAAAACATTTATTATGCTTAGT
GCTGGTTTAGGATTGTTTTTTTATGTTAATCAGA

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