I work for Professor Thomas von Zglinicki as a cell biologist/ microscopist, investigating the signalling pathways involved in induction and maintenance of the senescent phenotype in mammalian cells. Senescence is the irreversible cell cycle arrest which cells undergo as a consequence of irreparable DNA damage, such as shortened telomeres. A lot of my work in this lab has focussed on creating a reporter system for following the lifespan of sites of DNA damage in living cells using fluorescent reporter genes.
For this work we have several fluorescence microscopes, but the one I currently use most for the live cell work is our Zeiss LSM510 confocal, equipped with a temperature, humidity and gas controlled incubator from Solent Scientific.
For post image acquisition analysis and processing, we generally use Huygens deconvolution software (from SVI) and ImageJ. There's a particularly nice compiled set of ImageJ plugins put together by Tony Collins at McMaster University, Ontario.
Publications
These are listed on my ResearcherID page.
Contact
Dr. Glyn Nelson
Ageing Research Laboratories
Institute for Ageing and Health
Newcastle University
Campus for Ageing and Vitality
Newcastle Upon Tyne
NE4 5PL
Tel: +44 (0)191 248 1106
Fax: +44 (0)191 248 1101
glyn.nelson@ncl.ac.uk
